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Quantify neurite length and branch points in order to evaluate neural network stability over time – all inside your cell culture incubator. You can achieve automated, continuous, non-invasive imaging and measurement of neurite outgrowth with 96-/384-well assays. Quantitative Time-course data, measure GFP tube length formation (mm/mm2) Export metrics (tube length, tube area, branch points) to calculate EC50/IC50 values. Visualize kinetic plots in microplate views.
Neurotracker essenbiosciences software#
You can follow tube formation/inhibition in real time by acquiring and analyzing images of GFP positive endothelial cells using the integrated Angiogenesis software module. You can automatically monitor and quantify tumor spheroids information such as growth and health, in real time and inside your tissue culture incubator. The incuCyte® software enables accurate quantification of cell clustering over time using phase contrast images.ģD spheroid cell culture models are advanced tools to accelerate drug discovery. You can monitor proliferation and cell-cell clustering interactions without the need to label, in real time and without removing your cultures from the incubator. The IncuCyte® live-cell imaging and analysis system enables you to measure cell death based on cell membrane integrity, in real-time, in automated fashion. For more information on reagent and procedure, please go on Incucyte website. Two apoptotic pathways assay have been developed to be studied with the Incucyte software module, Caspase-3/7 and Annexin V, and can be studied simultaneously. The IncuCyte® Live-Cell Analysis System enables real-time and automated apoptosis assays inside your tissue culture incubator. The absence of proliferation is a simple marker for screening drugs that are hoped to induce cell death or senescence. The environmental stability that comes from being housed in a tissue culture incubator, along with automated analysis software makes the IncuCyte an attractive tool for performing compound screens. The instrument will automatically measure and report on wound closure.
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With a WoundMaker-96 reproducible wounds can be made in 96 well plates and imaged in the IncuCyte. The software comes bundled with analysis tool to measure proliferation and tube formation which can be displayed as plate maps making it easy to see instantly how these parameters change between different wells/treatments.Ī common method for assessing cell motility is to induce a scratch wound in a confluent monolayer of cultured cells. With a capacity of holding up to 6x 384-well plates the Inc圜yte has the potential to screen 2304 wells per experiment. Speed of acquisition is dependent upon the number of samples placed in the unit but is suited for intervals in the minutes to hours range. These metrics can be monitored during the course of imaging and are displayed graphically, allowing easy and quick interpretation of results. The acquisition software comes with automated analysis tools to aid with high throughput analysis, such as object counting, scratch wound closure, and confluence measurements. The imaging modes are phase contrast and two fluorescent channels (green and red). The system comes with a selection of versatile inserts allowing you to image your cells in a range of vessels. Housed inside a tissue culture incubator, the imaging module maintains the most stable imaging conditions possible ( 37C temperature, humidity and 5% CO2). The IncuCyte S3 is a widefield inverted live cell system engineered with long term high throughput imaging in mind. Katharina Gaus Light Microscopy Facility.
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Biological Resources Imaging Laboratory.
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